新型骨科材料SCPP对成骨细胞促血管化生长因子表达影响的研究

余喜讯

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新型骨科材料SCPP对成骨细胞促血管化生长因子表达影响的研究

材料科学与工程 | 更新时间：2023-11-14

- 新型骨科材料SCPP对成骨细胞促血管化生长因子表达影响的研究
- The study of the influence of SCPP on angiogenic growth factors expression of osteoblasts
- 工程科学与技术 2011年43卷第1期页码：219-225
- 作者机构：
  
  四川大学高分子科学与工程学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金，江苏省自然科学基金，四川省应用基础研究项目
- DOI：
  中图分类号：
- 纸质出版日期：2011，
  
  网络出版日期：2010-4-17，
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余喜讯.新型骨科材料SCPP对成骨细胞促血管化生长因子表达影响的研究[J].工程科学与技术,2011,43(1):219-225.

yu xi-xun. The study of the influence of SCPP on angiogenic growth factors expression of osteoblasts[J]. Advanced Engineering Sciences, 2011,43(1):219-225.
余喜讯.新型骨科材料SCPP对成骨细胞促血管化生长因子表达影响的研究[J].工程科学与技术,2011,43(1):219-225. DOI：

yu xi-xun. The study of the influence of SCPP on angiogenic growth factors expression of osteoblasts[J]. Advanced Engineering Sciences, 2011,43(1):219-225. DOI：

摘要

中文摘要: 在制备聚磷酸钙过程中掺入锶制备掺锶量为（1%，2%，5%，8%，10%）的新型骨科材料SCPP。研究SCPP对成骨细胞行为及其血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的影响，为阐明其促血管化机理提供实验依据。以成骨细胞与支架材料复合培养，采用XRD，MTT法，ALP活性检测，SEM观察，RT-PCR和ELISA技术等对其进行表征。结果表明：SCPP能够促进成骨细胞增殖，提高其ALP活性，上调成骨细胞VEGF和bFGF的mRNA和蛋白表达，与对照组CPP均有显著性差异（P<0.05），以8%SCPP最为明显。SCPP具有“促血管化作用”，是一个很有前景的骨组织工程支架或骨修复材料。

Abstract

Abstract:Strontium-doped calcium polyphosphate (SCPP) which were a series of calcium polyphosphate (CPP) respectively containing 0

10 mol% of Ca2+ replaced by Sr2+ used as a novel bone material were prepared. The influence of SCPP on cellular behavior and angiogenic factors which were vascular endothelial growth factor (VEGF) and basic fibroblastic growth factor (bFGF) of osteoblasts was investigated

which provides experimental basis for elucidating mechanism of SCPP promoting vascularization. Osteoblasts were cultured with SCPP scaffolds

XRD

MTT assay

quantitative detection of ALP activity

scanning electron microscopy (SEM) observation

Reverse Transcription-Polymerase Chain Raction (RT-PCR) analysis and Enzyme-linked Immunosorbent Assay (ELISA) were used to investigate. The results showed that

compared with the control

the cellular proliferation

ALP activity as well as the mRNA and protein expression of the angiogenic factors (VEGF and bFGF) of the SCPP group were significantly promoted (p<0.05)

and 8% SCPP showed the highest expression. SCPP is a potential material with “promoting vascularization”

and may serve as a promising scaffold for bone tissue engineering and bone implant materials.

关键词

掺锶聚磷酸钙血管化血管内皮生长因子碱性成纤维细胞生长因子

Keywords

Strontium-doped calcium polyphosphatevascularizationvascular endothelial growth factorbasic fibroblastic growth factor

references

Chen Yuanwei;Shi Guoqi;Ding Yulong,In vitro study on the infuence of strontium-doped calcium polyphosphate on the angiogenesis-related behaviors of HUVECs,Journal of Materials Science:Materials in Medicine,2008(7).

Marie P J,Effect of low doses of stable strontium on bone metabolism in rats,Mineral and Electrolyte Metabolism,1984(1).

Ciolfi VJ ；Pilliar R ；McCulloch C ；Wang SX ；Grynpas MD ；Kandel RA,Chondrocyte interactions with porous titanium alloy and calcium polyphosphate substrates.,Biomaterials ,2003, 24(26).

Waldman SD;Grynpas MD;Pilliar RM,Characterization of cartilaginous tissueformed on calcium polyphosphate substrates in vitro,Journal of Biomedical Materials Research

Colton C K,Implantable biohybrid artificial organs,Cell Transplantation,1995(4).

Choudhary S ；Wadhwa S ；Raisz LG ；Alander C ；Pilbeam CC,Extracellular calcium is a potent inducer of cyclo-oxygenase-2 in murine osteoblasts through an ERK signaling pathway.,Journal of Bone and Mineral Research ,2003, 18(10).

Yamamoto T. ；Kozawa O. ；Tanabe K. ；Akamatsu S. ；Matsuno H. ；Dohi S. Hirose H. ；Uematsu T.,1,25-dihydroxyvitamin D-3 stimulates vascular endothelial growth factor release in aortic smooth muscle cells: Role of p38 mitogen-activated protein kinase,Archives of Biochemistry and Biophysics ,2002, 398(1).

Hirschi K K;D Amore P A,Control of angiogenesis by the pericyte:molecular mechanisms and significance,Experientia Supplementum,1997.

Bouhadir KH;Mooney DJ,Promoting angiogenesis in engineered tissues,Journal of Drug Targeting

Herbertson A;Aubin JE,Cell sorting enriches osteogenic populations in rat bone marrow stromal cell cutures,Bone

Ozawa S. ；Kasugai S.,EVALUATION OF IMPLANT MATERIALS (HYDROXYAPATITE, GLASS-CERAMICS, TITANIUM) IN RAT BONE MARROW STROMAL CELL CULTURE,Biomaterials ,1996, 17(1).

Marie P J,Strontium ranelate:a physiological approach for optimizing bone formation and resorption,Bone,2006(Supp 1).

Sm Yang ；Sy Kim ；Sjlee,Tissue Response of calcium polyphosphate in beagle dog,Key Engineering Materials ,2002, 0(218/220).

Yang Liu;Wang Jian;Hong Jason,Synthesis and characterization of a novel polymer-ceramic system for biodegradable composite applications,Biomedical Materials Research,2003(3).

Baksh D;Dives J E,Three-dimensional matrices of calcium polyphosphates support bone growth in vitro and in vivo,Mat Sci:Mater Med,1998(12).

Tokuda H;Hirade K;Wang X,Involvement of SAPK/JNK in basic fibroblast growth factor-induced vascular endothelial growth factor release in osteoblasts,Endocrinology,2003(1).

Ryuto M;Ono M;Izumi H,Distinct protein kinase C isoforms mediate regulation of vascular endothelial growth factor expression by A2A adenosine receptor activation and phorbol esters in peochromocytoma PC12 cells,Biological Chemistry,1996.

Seghezzi G;Patel S;Ren C J,Fibroblast growth factor 2 (FGF2) induces vascular endothelial growth factor (VEGF) expression in the endothelial cells of forming capillaries:an autocrine mechanism contributing to angiogenesis,Cell Biology,1998(7).

Presta M;Dell\'Era P;Mitola S,Fibroblast growth factor/fibroblast growth factor receptor system in angiogenesis,Cytokine & Growth Factor Reviews

Hurley M M;Abreu C;Harrison J R,Basic fibroblast growth factor in hibits type 1 collagen gene expression in osteoblastic MC3T3-E1 cells,Biological Chemistry,1993.

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