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工程科学与技术:2013,45(4):176-180
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高效氯氰菊酯降解菌的分离与鉴定及其降解条件优化
(1.四川大学 轻纺与食品学院;2.西华大学 生物工程学院)
Isolation and Characterization of Beta-cypermethrin Degrading Strain and its Condition Optimization
(1.College of Light Industry and Food,Sichuan Univ.;2.School of Bioengineer,Xihua Univ.)
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投稿时间:2013-01-25    修订日期:2013-04-08
中文摘要: 从长期施用高效氯氰菊酯农药的菜园土壤中,分离到一株能降解高效氯氰菊酯的细菌GCN019。经生理生化和16S rDNA分析,将菌株GCN019鉴定为荧光假单胞菌(Pseudomonas fluorescens)。应用Plackett-Burman 试验设计确定了影响该菌株降解高效氯氰菊酯的主要因素,利用响应面分析法优化了其降解条件。结果表明,降解体系中高效氯氰菊酯浓度和接种量及初始pH是影响其降解的主要因素;优化条件下高效氯氰菊酯的降解率可达到73.94%,与所建立的模型预测值(74.30%)相吻合。
Abstract:Strain GCN019 with high ability to degrade beta-cypermethrin was isolated from garden soil which contaminated by beta-cypermethrin for a long term.The strain was identified as Pseudomonas fluorescens based on the results from its physiological-biochemical characteristics and phylogenetic similarity of 16S rDNA gene sequences.Then,the main factors influencing beta-cypermethrin degradation were investigated by using Plackett-Burman design,and the degradation condition was optimized with response surface methodology.It was found that beta-cypermethrin concentration,inoculum volume and initial pH value were the major factors influencing beta-cypermethrin degradation.In addition,the degradation rate of beta-cypermethrin under the optimized condition reached 73.94%,which was similar to the predictive value from response surface model(74.30%).
文章编号:201300066     中图分类号:    文献标志码:
基金项目:国家自然科学基金资助项目(21072137);成都市科技攻关课题资助项目(11DXYB208NC)
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唐洁,姚开,贾冬英,迟原龙,李清平.高效氯氰菊酯降解菌的分离与鉴定及其降解条件优化[J].工程科学与技术,2013,45(4):176-180.
Tang Jie,Yao Kai,Jia Dongying,Chi Yuanlong,Li Qingping.Isolation and Characterization of Beta-cypermethrin Degrading Strain and its Condition Optimization[J].Advanced Engineering Sciences,2013,45(4):176-180.