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工程科学与技术:2010,42(2):213-218
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低分子量壳聚糖的制备及其对成纤维细胞增殖的影响
(1.四川大学 高分子科学与工程学院,四川 成都 610065;2.四川大学 高分子科学与工程学院)
Preparation of Low Molecular Weight Chitosans and Their Effect on Proliferation of Fibroblasts
(1.School of Polymer Sci. and Eng., Sichuan Univ., Chengdu 610065, China;2.School of Polymer Sci. and Eng., Sichuan Univ.)
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投稿时间:2009-01-15    修订日期:2009-04-02
中文摘要: 采用H2O2/CH3COOH降解体系和梯度乙醇溶液分级的方法制备了一系列分子量不同,分散度较低的低分子量壳聚糖。红外光谱和13C核磁共振谱表明制备的低分子量壳聚糖化学结构与原料相似,无明显变化。X射线衍射谱显示:随着分子量的降低,其晶型由原料的“L-2 polymorph”晶型向“annealed polymorph” 和“tendon hydrate polymorph” 的混合晶型转变,最后趋近于无定形。体外细胞培养实验表明:在较低浓度下,制备的低分子量壳聚糖具有促进成纤维细胞增殖的作用;而壳聚糖的单体氨基葡萄糖在1mg/ml浓度下具有细胞毒性。
中文关键词: 低分子量  壳聚糖  成纤维细胞
Abstract:A series of low molecular weight chitosans (LMWCS) with different molecular weight and lower polydispersity were prepared by the degradation of high molecular weight chitosan with H2O2/CH3COOH and gradient ethanol solution treatment. Fourier transform infrared spectra (FTIR)and 13nuclear magnetic resonance spectroscopy (13CNMR) analysis showed that the structure of the prepared LMWCS was similar to that of original chitosan without any significant change. X-ray diffraction (XRD) measurements indicated that, with the decrease of the molecular weight, the pattern transformed from "L-2 polymorph"(original chitosan) to a mixture of "annealed polymorph" and "tendon hydrate polymorph", and finally tended to amorphous. Cell culture experiments in vitro suggested that, the prepared LMWCS, in low concentrations, promoted the proliferation of fibroblasts; however, the monomer of chitosan (D-glucosamine, GlcN) had cytotoxicity in the concentration of 1 mg/ml.
文章编号:200900055     中图分类号:    文献标志码:
基金项目:国家自然科学基金资助项目( 30870614);四川大学大学生创新实验国家资助项目
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田猛,沈磊,李东旭,张云婷,孟令蝶,杨明珠,余喜讯,张小华,万昌秀.低分子量壳聚糖的制备及其对成纤维细胞增殖的影响[J].工程科学与技术,2010,42(2):213-218.
Tian Meng,Shen Lei,Li Dongxu,Zhang Yunting,Meng Lingdie,Yang Mingzhu,Yu Xinxun,Zhang Xiaohua,Wan Changxiu.Preparation of Low Molecular Weight Chitosans and Their Effect on Proliferation of Fibroblasts[J].Advanced Engineering Sciences,2010,42(2):213-218.