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工程科学与技术:2008,40(5):94-98
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不同种类微生物利用制酒废水产生微生物 絮凝剂及其性能比较
(四川大学 建筑与环境学院, 四川 成都 610065)
The Proparation and Activity Comparison of Bioflocculant from Alcohol Wastewater Using Different Microorganisms
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投稿时间:2008-01-20    
中文摘要: 对蜡状芽孢杆菌(Bacillus cereus)、膜璞毕赤酵母(Pichia membranifaciens)及其两株菌的混合菌(HXJ-1)利用制酒废水产生微生物絮凝剂进行了研究。结果表明,HXJ-1与单菌株相比表现出明显的优势:其一,可以直接利用酸性制酒废水产生微生物絮凝剂,不必调节pH;其二,产絮周期大大缩短,由单菌株的48 h缩短至24 h;另外,能利用的制酒废水浓度高、外加氮源少。HXJ-1利用制酒废水产生微生物絮凝剂既降低了絮凝剂的生产成本,同时又降低了制酒废水中有机物浓度,达到了以废治废目的。HXJ-1的最佳絮凝剂产生条件为:废水COD浓度为12000 mg/L,C/N比为20∶1, 相对接种量10% (V/V, 菌体浓度: 108 个/L),初始pH 3.6 (制酒废水自然pH) , 摇床转速为120 r/min。
Abstract:Bacillus cereus, Pichia membranifaciens and mix strains (named as HXJ-1) consisting of these two strains were used to produce bioflocculant by utilizing alcohol wastewater. The result showed that HXJ-1 has apparent advantage compared with single strain. First, HXJ-1 could use acid alcohol wastewater to produce bioflocculant directly, no need to adjust original pH; second, the production period of HXJ-1 is much shorter than that of single strain, from 48 hours reducing to 24 hours. On the other hand, HXJ-1 could adapt higher COD concentration and input less N source. Not only the cost of bioflocculant production, but also the organic concentration in alcohol wastewater is reduced by HXJ-1 utilizing alcohol wastewater to produce bioflocculant, so this method realizes the purpose of “use waste to treat waste”. Optimal bioflocculant production conditions of HXJ-1 were as follows: COD concentration of wastewater at 12000 mg/L, C/N ratio at 20∶1, relative volume of inoculation: 10% (V/V) with strain concentration: 108 /L, initial pH of culture medium at 3. 6 (nature pH of alcohol wastewater), rotate rate at 120 r/min and the culture temperature at 30 ℃。
文章编号:20080517     中图分类号:    文献标志码:
基金项目:四川省应用基础项目(05JY029-026-2)
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张帆,蒋文举,王向东.不同种类微生物利用制酒废水产生微生物 絮凝剂及其性能比较[J].工程科学与技术,2008,40(5):94-98.
.The Proparation and Activity Comparison of Bioflocculant from Alcohol Wastewater Using Different Microorganisms[J].Advanced Engineering Sciences,2008,40(5):94-98.