Abstract:Bacillus cereus, Pichia membranifaciens and mix strains (named as HXJ-1) consisting of these two strains were used to produce bioflocculant by utilizing alcohol wastewater. The result showed that HXJ-1 has apparent advantage compared with single strain. First, HXJ-1 could use acid alcohol wastewater to produce bioflocculant directly, no need to adjust original pH; second, the production period of HXJ-1 is much shorter than that of single strain, from 48 hours reducing to 24 hours. On the other hand, HXJ-1 could adapt higher COD concentration and input less N source. Not only the cost of bioflocculant production, but also the organic concentration in alcohol wastewater is reduced by HXJ-1 utilizing alcohol wastewater to produce bioflocculant, so this method realizes the purpose of “use waste to treat waste”. Optimal bioflocculant production conditions of HXJ-1 were as follows: COD concentration of wastewater at 12000 mg/L, C/N ratio at 20∶1, relative volume of inoculation: 10% (V/V) with strain concentration: 108 /L, initial pH of culture medium at 3. 6 (nature pH of alcohol wastewater), rotate rate at 120 r/min and the culture temperature at 30 ℃。