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工程科学与技术:2007,39(4):68-72
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从白鲜皮中同时制备梣酮、白鲜碱、 黄柏酮单体的方法
(1.四川大学 轻工与食品学院,四川 成都 610065;2.成都中医药大学 药学院,四川 成都 610075)
The Simultaneous Preparation of Fraxinellone, Dictamine andObacunone from Dictamnus Dasycarpus. Turcz
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投稿时间:2007-01-23    
中文摘要: 以HPLC测定计算的梣酮、白鲜碱、黄柏酮3种单体的总单体得率为指标,探讨了提取溶媒、提取方式、提取时间、液料比和提取次数对提取效果的影响,并运用正交试验对提取工艺进行优选;提取得到的浸膏进行一次硅胶柱层析和重结晶即分离纯化出3种单体;单体经波谱方法进行结构鉴定,经TLC和HPLC进行纯度检查和含量测定。结果显示,最佳的提取工艺为加入药材重量6倍的乙酸乙酯回流提取3次,每次1 h;分离纯化后的3种单体纯度高,用其作为对照品对白鲜皮药材进行含量测定结果满意。实验表明提取工艺及分离方法能同时制备3种单体,方法简
中文关键词: 梣酮  白鲜碱  黄柏酮  白鲜皮  提取  正交试验
Abstract:The effects of extraction solvent, extraction method, extraction time, ratio of solvent to raw material and number of times on the total yield of fraxinellone, dictamine and obacunone were studied, and the extraction condition were optimized by orthogonal experiment. By the method of one time chromatography and recrystallization, three compounds were separated from the residue. The structures of the compounds were elucidated on the basis of spectral analysis, and the purity and concentration was detected by TLC and HPLC. The results showed that the optimum extraction condition was that the raw material was reflux extracted by EtoAc (1 h×3 times), with ratio of solvent to raw material 6∶1. These compounds can be used as reference substance for the content determination. The method established can simultaneously separated fraxinellone, dictamine and obacunone from Dictamnus dasycarpus Turcz., with simple and rapid procedure, high purity and yield of the three compounds.
文章编号:20070414     中图分类号:    文献标志码:
基金项目:国家科技部中小企业创新基金资助项目(04C26225100815)
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李翔,张新申,邓赟.从白鲜皮中同时制备梣酮、白鲜碱、 黄柏酮单体的方法[J].工程科学与技术,2007,39(4):68-72.
.The Simultaneous Preparation of Fraxinellone, Dictamine andObacunone from Dictamnus Dasycarpus. Turcz[J].Advanced Engineering Sciences,2007,39(4):68-72.