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工程科学与技术:2007,39(1):74-77
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固定化酶乙醇荧光毛细分析法的研究
(1.四川大学 化学工程学院,四川 成都 610065;2.四川大学 华西基础医学与法医学院,四川 成都 610041)
Research on the Immobilized-enzyme Alcohol Fluorescence Capillary Analysis
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投稿时间:2005-11-22    
中文摘要: 提出了固定化酶乙醇荧光毛细分析(IE-EFCA)的新方法和乙醇毛细生物反应器(ECBR)。ECBR用交联剂将乙醇脱氢酶和辅酶I固定于毛细管内壁制成,可重复使用。IE-EFCA法的实验条件:激发波长350 nm、发射波长459 nm、反应时间20 min、反应温度40 ℃、磷酸缓冲液浓度0.1 mol/L(pH 7.5)、NAD+浓度5 mmol/L、ADH固定化酶的浓度10 U/L;乙醇的线性范围2.0~15 g/L,r>0.9958,检出限为1.1 g/L,RSD<1.7%。IE-EFCA法重现性好、操
中文关键词: 荧光毛细分析  NADH  乙醇  测定
Abstract:A new method for the determination of ethanol based on the immobilized enzyme fluorescence capillary analysis (IE-EFCA) was proposed. Alcohol dehydrogenation (ADH) was immobilized in inner surface of a capillary. After NAD+ as an oxidizer was mixed with the samples solution, the determination was conducted to the mixed solution in the capillary at 350 nm of excited wavelength and at 459 nm of emission wavelength. The experimental conditions for ethanol determination are as follows: reaction time is 20 min, temperature is 40 ℃, the concentrations of phosphate buffer solution (pH 7.5) and NAD+ are 0.1 mol/L and 5 mmol/L, respectively; immobilization concentration of ADH is 10 U/L. The linear range for the determination of ethanol is 2.0~15.0 g/L, r>0.9958, the detection limit of ethanol is 1.1 g/L, RSD is 1.9%. IE-EFCA method is applicable for the determination of the alcohol samples in medicine, industry, grocery, and environment.
文章编号:20070115     中图分类号:    文献标志码:
基金项目:四川大学214振兴计划基金资助项目(0082204127098)
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李永生,高秀峰.固定化酶乙醇荧光毛细分析法的研究[J].工程科学与技术,2007,39(1):74-77.
.Research on the Immobilized-enzyme Alcohol Fluorescence Capillary Analysis[J].Advanced Engineering Sciences,2007,39(1):74-77.