Isolation and Characterization of Beta-cypermethrin Degrading Strain and its Condition Optimization

doi:10.15961/j.jsuese.2013.04.002

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Isolation and Characterization of Beta-cypermethrin Degrading Strain and its Condition Optimization

更新时间：2023-11-14

- Isolation and Characterization of Beta-cypermethrin Degrading Strain and its Condition Optimization
- Advanced Engineering Sciences Vol. 45, Issue 4, Pages: 176-180(2013)
- 作者机构：
  
  1. 四川大学轻纺与食品学院西华大学生物工程学院
  2. 四川大学轻纺与食品学院
- 作者简介：
- 基金信息：
- DOI：10.15961/j.jsuese.2013.04.002
  CLC：
- Published：2013，
  
  Published Online：8 April 2013，
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唐洁,姚开,贾冬英,迟原龙,李清平.高效氯氰菊酯降解菌的分离与鉴定及其降解条件优化[J].工程科学与技术,2013,45(4):176-180.

Isolation and Characterization of Beta-cypermethrin Degrading Strain and its Condition Optimization. [J]. Advanced Engineering Sciences 45(4):176-180(2013)
唐洁,姚开,贾冬英,迟原龙,李清平.高效氯氰菊酯降解菌的分离与鉴定及其降解条件优化[J].工程科学与技术,2013,45(4):176-180. DOI： 10.15961/j.jsuese.2013.04.002.

Isolation and Characterization of Beta-cypermethrin Degrading Strain and its Condition Optimization. [J]. Advanced Engineering Sciences 45(4):176-180(2013) DOI： 10.15961/j.jsuese.2013.04.002.

摘要

中文摘要: 从长期施用高效氯氰菊酯农药的菜园土壤中，分离到一株能降解高效氯氰菊酯的细菌GCN019。经生理生化和16S rDNA分析，将菌株GCN019鉴定为荧光假单胞菌（Pseudomonas fluorescens）。应用Plackett-Burman 试验设计确定了影响该菌株降解高效氯氰菊酯的主要因素，利用响应面分析法优化了其降解条件。结果表明，降解体系中高效氯氰菊酯浓度和接种量及初始pH是影响其降解的主要因素；优化条件下高效氯氰菊酯的降解率可达到73.94%，与所建立的模型预测值（74.30%）相吻合。

Abstract

Abstract:Strain GCN019 with high ability to degrade beta-cypermethrin was isolated from garden soil which contaminated by beta-cypermethrin for a long term.The strain was identified as Pseudomonas fluorescens based on the results from its physiological-biochemical characteristics and phylogenetic similarity of 16S rDNA gene sequences.Then

the main factors influencing beta-cypermethrin degradation were investigated by using Plackett-Burman design

and the degradation condition was optimized with response surface methodology.It was found that beta-cypermethrin concentration

inoculum volume and initial pH value were the major factors influencing beta-cypermethrin degradation.In addition

the degradation rate of beta-cypermethrin under the optimized condition reached 73.94%

which was similar to the predictive value from response surface model(74.30%).

关键词

高效氯氰菊酯生物降解荧光假单胞菌条件优化

Keywords

beta-cypermethrinbiodegradationpseudomonas fluorescenscondition optimization

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