Abstract:Considering of the distortion caused by the accumulative error in multiple core fluorescence image stitching

we proposed a global optimization methods based on L-M(Levenberg—Marquardt) algorithm. In order to achieve global registration

which can minimize the sum of error distance for all feature point after spherical transforming in all image

we based on the initial parameters of the spherical projection for each image and used L-M algorithm to do iterative calculation;during the iterative calculation

the damping parameter μ will be real-time adjusted due to the gain ratio δ so that we can continuously update the iteration direction and the size of step

find the optimal set of projection parameter. The algorithm is applied to the multiple core fluorescence images stitching

solved the problem that the distortion of stitched image caused by the accumulative error

to obtain satisfactory results.