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工程科学与技术:2012,44(4):169-175
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负压双管线流动注射离子选择电极法同时测定血清K+、Na+、Cl-、Ca2+
(1.四川大学 化工学院;2.深圳大学 信息学院;3.四川大学 基础医学与法医学院)
A Negative-pressure Double-lines Flow-injection Ion-selective Electrode Method forSimultaneous Determination of K+、Na+、Cl-、Ca2+ in Serums
(1.School of Chem. Eng., Sichuan Univ.;2.School of Info. Technol., Shenzhen Univ.;3.West China School of Preclinical and Forensic Medicine, Sichuan Univ.)
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投稿时间:2011-07-27    修订日期:2012-03-08
中文摘要: 为了简化装置结构、降低仪器成本、提高分析系统的稳定性和抗干扰能力,推出一种全新的负压式双管线流动注射离子选择电极分析方法,并对该方法相关的载液组成及流量、载流酸度调节液的组成及流量,反应盘管长度等进行了优化,最后将其成功用于血清K+、Na+、Cl-、Ca2+的同时测定。方法的相对标准偏差<1.3% (n=11),回收率为96%~104%,分析速度为480 检出/h,样品耗量仅为45 μL/次;对血清K+、Na+、Cl-、Ca2+的同时测定范围分别是2.0~24.0、102~252、20~300、0.70~8.0 mmol·L^-1,此测定范围涵盖了正常人血清中的电解质浓度。
Abstract:In order to simplify the device configuration, reduce the cost, improve the instrument stability and anti-interference ability, a new type of double-line flow-injection ion-selective electrode method was proposed, and determination conditions of the method, such as the carrier composition and flow rate, acidity adjusting liquid composition and flow rate, and reaction coil length were optimized. The method was successfully used in simultaneous determinations of K+、Na+、Cl-、Ca2+in serums. Linear ranges of the method were in 2.0~24.0 mmol·L^-1for K+, 102~252 mmol·L^-1for Na+, 20~300 mmol·L^-1for Cl- and 0.7~8.0 mmol·L^-1 for Ca2+, respectively. The relative standard deviation was less than 1.3% (n=11), the recoveries obtained by adding K+,Na+,Cl- and Ca2+in serum samples were in range 96%~104%, the analysis speed was 480 detections/h, and the consumption of the sample was only 45 μL per time. These determination ranges were in normal ranges of human serum’s electrolyte concentrations.
文章编号:201100688     中图分类号:    文献标志码:
基金项目:四川大学214人才引进振兴计划基金资助项目(0082204127067)
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李永生,张会生,周磊,赖振尧,高秀峰.负压双管线流动注射离子选择电极法同时测定血清K+、Na+、Cl-、Ca2+[J].工程科学与技术,2012,44(4):169-175.
Li Yongsheng,Zhang Huisheng,Zhou Lei,Lai Zhenyao,Gao Xiufeng.A Negative-pressure Double-lines Flow-injection Ion-selective Electrode Method forSimultaneous Determination of K+、Na+、Cl-、Ca2+ in Serums[J].Advanced Engineering Sciences,2012,44(4):169-175.